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Cryo-EM

Principal Key Products About Key Products
Mitegen
Cryo EM Starter Kits The MiTeGen Cryo-EM Lab Tool Kits are designed to complement the Thermo Fisher Scientific™ Vitrobot as
well as Thermo Fisher Scientific™ Cryo-EM microscopes such as the Krios G4, Glacios and Tundra. The kits
provide many of the components that are needed for cryo-EM sample preparation as well as sample storage
and transport.
Cryo-EM Grid Storage
Pucks
MiTeGen’s Cryo-EM Grid Storage and Shipping Systems (kits) are designed to organize, store, track, and
ship your samples on grids for single particle analysis and other Cryogenic Electron Microscopy (CryoEM)
applications with ease and efficiency. They offer several systems (kits) ranging from the Basic System
which is ideal for small labs or those just starting to use CryoEM to the TW35-10 Complete System which
is designed for large labs.
Cryo-EM Grid Boxes &
Accessories
MiTeGen provides Specimen grid boxes for storing or transferring cryogenic TEM specimen grids. Round
designs fit MiTeGen Cryo-EM Grid Box Storage Pucks, Four diamond shaped slots, Non-static cover is held
in place with stainless steel screw, which is tapped in the center of the box, Boxes that fit the FEI
Vitrobot™, Gatan 626™, Gatan 3500™
Cryo-EM Vitrification
Tools
MiTeGen provides Tools and Equipment for improving vitrification of Cryo-EM grids used in single
particle analysis e.g Plunge Cooler, Ethane Cryostat System, Ethane condenser, Plunge Cooler Accessories
etc
Cryo-EM Vitrification &
Detergents
MiTeGen offers reagents for improving vitrification of Cryo-EM grids used in single particle analysis
e.g Cryo-EM Vitrification Starter Kit & Amphipol A8—35
Cryo-EM Cryogenic Dewars &
Shippers
MiTeGen provides Cryogenic Dewars, Shippers, Refrigerators for Cryo-EM sample cooling, storage, and
transport e.g Custom Designed Foam Dewar, Cryogenic Refrigerators, Dewars & Shippers, Cryo-EM Puck Dewar
& Insert, Spearlab Foam Dewars
Cryo-EM Instrumentation MiTeGen offers the instruments you need to prepare and plunge cool your grids for cryo-EM e.g Custom
Manual Plunge Cooler & Sample Preparation Chamber, Manual Plunge Cooler & Precision Ethane Cryostat,
Glow Discharge Plasma Cleaner & Accessories, Economy Dry Oxygen Service Pump, Vacuum gauge & Digital
meter, Quartz Sample Tray, MiTeGen V81 Stereo Microscope etc
Nanosoft LLC Grid Boxes Nanosoft offers Grid boxes with a variety of innovative, patented designs optimized for any type of
cryoEM workflow. Grid Box Lids with different geometries and materials for different applications, and
tools to help with handling grid boxes e.g Bulk Storage Grod Boxes, 8-Pocket Grid Box, Metal Universal
HPF & Grid Box, Hybrid Grid Box, 12 Pack Metal Pin Lids, Pin Lid Manipulator Tool, Metal Pin Lid, Pin
Lid, CryoEM Grid Box Tweezers etc
Vitrification Tools Nanosoft provides tools that improve the efficiency and ease of the plunge freezing Vitrification
workflow, with the Vitrobot or with manual, custom plungers, providing higher quality grids e.g Ethane
Temperature Controlled Vitrobot Dewar, Cryo-Tweezers, Igloo, Vitrification Dewar Incl Metal parts, Pin
Lid Manipulator Tool, Spider/Grid Box Ring/Ethane Cup Vitrobot Dewar Parts, Foam Ring, Ethane Condenser,
Quick Lid etc
Clipping & Autogrid
Rings
Products that enable the clipping of cryoEM grids for assembly into autogrids for insertion into Thermo
Fisher cryoEM autoloaders, the manipulation of autogrids and their inspection to ensure quality e.g 100
pack Autogrid Rings & C Clips, 100-Pack C-Clip, Clipping Station w/8/16 clipping tools, Autogrid
Tweezer, 4-Pack Clipping tool, Reverse Action Autogrid Tweezer, Clipping Tool Rack, Cassette Tweezer &
Autogrid Inspection Tool
Cryo FIB/CLEM/ET
Workflows
Aquilos/Hydra Extended Operation System & Linkam/Leica CryoCLEM cartridge adaptor
Cryo Stages & Holders Custom cryo-holders and modifications to existing holders to improve ease of workflow, minimize
handling, and reduce risk of damaging samples. Furthermore, accessories and tools necessary for
operation of current cryo-holders e.g Cassette Tweezer, Linkam/Leica CryoCLEM cartridge adaptor, Gatan
G26 Controller cable, Gatan 626 Transfer station lid, Gatan 626 Transfer station lid plug, Gatan 626 60
deg Clipring tool, Gatan 626 60 degrees clipring
Foam Dewars Foam Dewars used for any handling and general use of liquid cryogens and for cryoEM workflow steps
required under LN2 e.g Spearlab Mini Vessel, Spearlab Small Vessel, Spearlab Large Vessel, Spearlab
Standard Vessel, Spearlab Tall Dewar
Ted Pella Support Grids or Substrates for
Transmission Electron Microscopy
Quantifoil® Holey Carbon Film is a perforated support foil with a p re-defined hole size, shape and
arrangement. They are offered with circular and square, orthogonally arranged holes. The film is mounted
on a standard Ø3mm Cu, Au, or Ni TEM grid with 200, 300 or 400 mesh. They have advantages in electron
microscopy (EM) or low-energy electron point source (LEEPS) microscopy when compared with conventional
holey film. The final thickness of the foil is about 20nm, although only 10nm of carbon is evaporated
onto the plastic

The UltrAufoil grids : One of the major problems when performing electron cryo-microscopy (cryo-EM) is
specimen movement from beam irradiation during imaging, which results in blurred, low resolution images.
One cause of excessive specimen drift is the use of carbon foils on metal (Cu) grids, which can move up
to 400Å parallel to the beam. The UltrAuFoil® grids were specifically developed for cryo-EM, to reduce
the movement of cryo-specimens during imaging. The UltrAufoil® grids are a 200 or 300 mesh gold grid
with a 400-500Å thick polycrystalline gold foil, which have a regular square array of micrometer-sized
circular holes. These grids are similar to Quantifoil® Grids except that the UltrAuFoil® grids use gold
foil instead of a carbon foil and are less fragile. Similar to the carbon supports, the UltrAuFoil®
grids can be made hydrophilic using low-energy plasma. The UltrAuFoil® grids can improve your image
quality by reducing radiation-induced motion without the use of sophisticated drift correction or
hardware.

Cryo Grid Boxes & Handling Tools The Cryo Grid Boxes are used for storing and transferring vitrified Cryo-EM specimens prepared with
cryo-devices like the FEI Vitrobot, Gatan CP3, Leica EM 6P, as well as other cryo vitrification systems.
Each box holds 4 grids and is available in two colours and three versions: with clear lid, pin type lid,
and without lid. All boxes have a UNC 5/40 thread in the centre. On versions with clear lids, the lid is
held in place with a stainless steel screw.

Compatability Round: FEI Vitrobot, Leica EM 6P, Gatan CP3, 626, CT3500, Square: Gatan CP3 & CT3500

Handling Tools e.g Multicolor Cryo Grid Box Handling Rod, Gripper Tool for Cryo Grid Box, Pin Lid
Manipulator Tool for Cryo Grid Box & Cryo Grid Box Metal Gripper Tool

Cryo-EM Tweezers Autogrid Tweezers for ThermoFisher/FEI, Tweezers Assembly for Vitrobot Mark IV, III, II & I, Dumont
Electronic Tweezers, PELCO Pro ESD safe Epoxy Precision Tweezers, PELCO Egronomic, ESD safe soft grip
Tweezers, PELCO ESD Safe Ergonomic Tweezers, Pelco Tweezer Trap
Cryo-EM Instrumentation PELCO EasiGlow™ Cryo-EM Glow Discharge Set

This is a bundle including all items needed for a typical glow discharge setup in a cryo-EM lab. This
set includes, in addition to a PELCO easiGlow™ unit, a specially matched vacuum pump with all
accessories, a rubber gasket to protect the glass chamber edge, as well as one PELCO® Grid Holder Block,
a handy alternative to putting your grids on glass slides.

CRESSINGTON 208C, Hight Vacuum Turbo Carbon Coater

The 208C High Vacuum Carbon Coater from Cressington offers high quality carbon coating ideal for making
carbon support films for TEM applications and other demanding methods such as FE-SEM, EDS/WDS, STEM,
EBSD, and EPMA